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Affinity Chromatography and Its Importance in LPLC
Affinity chromatography is known for its extraordinary specificity, and when applied through LPLC, it becomes one of the most powerful purification methods available. Using ligands that specifically bind to target molecules, affinity chromatography can purify biomolecules in a single step. This is particularly useful for antibody purification, tagged protein isolation, and purification of enzymes.
LPLC affinity systems are commonly used in biotechnology labs where researchers rely on mild conditions to maintain the bioactivity of proteins. After binding, elution is typically achieved through pH changes, competitive ligands, or salt gradients. This simplicity, combined with high purity yields, makes affinity chromatography a preferred LPLC technique.
